Dear Julia: You need complete the B cell antigens to characterize the B lineage with CD22 (membrane and cytoplasmic), CD79a and TdT. If are negative, probably the blast cells are undifferentiated and you will conclude the diagnostic of undifferentiated acute leukemia. Look at the morphology and the cytochemistry. There are very important in this case. Best wishes Mariano >Dear Flow-ers, >We got a bone marrow sample from a 5-year-old boy who has anaemia, >thrombopenia and leukopenia, returning febrile status, leg pain. >We found that only 10% of bone marrow cells expressed CD45. 90% of >the blast-like cells were HLA-DR+ and CD10+, but they do not express >CD19. The percentage of CD34+ cells are also very low (0.1%). We >could not detect neither CD33 or CD13. 5% of the cells were positive >for CD3, CD5 or C7. >What kind of malignancy can this be? >I would appreciate any comments. >Julia > >Julia B. Szeberenyi M.D >Dept. of Genetics, Cell-and Immunobiology >Laboratory of Flow Cytometry >Semmelweis University of Medicine >H-1089 Budapest, Nagyvarad ter 4 >Hungary >email: szebjul@net.sote.hu >fax:+36-1-303-69-68 Dr. Mariano P. Scolnik Inmunologia Oncologica Academia Nacional de Medicina Pacheco de Melo 3081 (1425) Buenos Aires Argentina mscolnik@interlink.com.ar
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