Dear Michael and Yanhui,
As you might expect, WinMDI does read valid FCS files, so any unreadable CellQuest
generated listmode file has somehow been corrupted during transfer. This is usually by
using ASCII mode in a ftp transfer instead of BINARY. The "new" files get an
extra carriage return embedded that will cause errors. My guess is you only need to
be transfer them again in BINARY mode. If this doesn't work, email a note to me and
we'll upload one to debug the problem...
Joe
Michael Ormerod wrote:
> I had a request for help from Yanhui Yang in China in analysing DNA histograms.
>
> I suggested using WINMDI to create DNA histograms and then Cylchred to analyse them
> (see below). However, he was unable to read his data files using WINMDI.
>
> I have never had a problem reading BD files supplied to me by other people.
> I do not have direct access to a Cellquest system to explore this directly.
> Can anyone help?
>
> Thanks,
>
> Michael Ormerod
>
> -------------Forwarded Message-----------------
>
> From: Yanhui YANG, INTERNET:yhyang@jzw.cern.ac.cn
> To: Michael Ormerod, Michael_Ormerod
>
> Date: 17/01/00 23:09 PM
>
> RE: Re: help
>
>
> Dear Dr.Ormerod,
>
> Thank you very much for your kindness and the information provided. I've downloaded
> those free softwares and tried my data on them. But unfortunately, there was
> something wrong when the downloaded software WinMDI reading my data files. It was
> said that the files were not FCS files. Actually, the data were originally acquired
> in Japan by a FACSCalibur FCM with a software of CellQuest running on Mac, and then
> transfered into PC readable format for the reason that we do not have a Mac here. I
> wonder if it is this transfermation cause the error in reading the files? If so, how
> can I make the analyze feasible? I'll be very grateful if you would provide further
> help.
>
> Thanks again and sorry for the bother.
>
> Best wishes
>
> Sincerely,
> Yanhui
>
> In 00-1-17 17:00:00 you wrote:
> >If you have your data in a listed data file in FCS format (used by all
> >modern flow cytometry systems) you can produce histograms in FCS format
> >using WINMDI.
> >
> >You can then analyse these files using Cylchred.
> >
> >Both these programs are available free on the Internet.
> >
> >WINMDI is available from http://facs.scripps.edu.
> >
> >Cylchred is available from http://www.uwcm.ac.uk/uwcm/hg/hoy/software.html.
> >
> >
> >I hope that this is of help to you.
> >
> >Michael Ormerod
> >34 Wray Park Road
> >Reigate RH2 ODE
> >Telephone: +44 (0)1737 241726
> >PLEASE NOTE NEW FAX & MOBILE NUMBERS
> >FAX: +44 (0)1737 226736
> >Mobile telephone: 07802 293242
> >Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
>
>
--
Joseph Trotter, Director
Flow Cytometry Core Facility
The Scripps Research Institute
10550 North Torrey Pines Road
La Jolla, CA 92037
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:03 EST