I have a client that is staining with 7-nitrobenz-2-oxa-1,3-diazole(NBD)C6-ceramide. According to the information he supplied to me, ie the Molecular Probes catalog, the fluorescence of NBD is dependent on the solvent. He ran the samples before in PBS and saw very little signal, so he wants to run samples in 100% MeOH. This doesn't sound like a good idea to me for a number of reasons. Is there a way to make this work? I would most appreciate any info on this subject. David McFarland Howard Hughes Medical Institute Flow Cytometry Facility Vanderbilt University Medical Center
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