Re: Flow Check Particles

From: Ron (rondudek@miltenyibiotec.com)
Date: Thu Jan 13 2000 - 16:35:49 EST


Neo Sim Yee, Lezlie writes:

>Dear all,
>
>I have an enquiry with regards to the flow check paticles that are used for
>assessing the optical alignment of the flow cytometer.
>
>In our work, we are dealing with bacteria of a size range as small as 0.5um.
>The flow check we are currently using is the 10um Flow Check Fluorosphere
>from Coulter. Noting the relatively large difference in size of the flow
>check particles and the cells that we are analysing, does this introduce a
>significant amount of error in the sense that the instrument may not be
>optimally aligned fot the analysis of such small paticles?
>
>Thank you for your help.
>
>Kind Regards
>
>Neo Sim Yee, Lezlie
>Department of Civil Engineering
>National University of Singapore

To which I offer these 1.99 cents:

In my experience analyzing bacteria with an arc lamp cytometer (BRYTE 
HS), aligning a cytometer with 1.5 micron beads was a successful 
procedure for optimally analyzing bacteria, including 0.5 micron 
sized bugs.

So perhaps it is "linear" down to the sub-micron range..

 From empirical experience, forward and side scatter determined size 
of bacteria on the arc lamp BRYTE, did not always correlate to 
forward and side scatter determined size of particles.  "Beads are 
not bugs" was our motto.  Didn't get to the T-shirt stage, though.
Regards,
Ron

Ronald P. Dudek
Clinical Products Business Manager
Miltenyi Biotec, Inc.
251 Auburn Ravine Rd.
Suite 208
Auburn, CA 95603

Phone (800) 367-6227
Fax (530) 888-8925



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