Dear Neo, I have seen very noticable differences in the alignment when using 10um Flow Check beads versus .76um Polyscience Fluoesbrite beads. I also have had to run .5um cells (they were phytoplankton in my case) and I definitley get a much better alignment for these small cells when I use the .76 um beads. This is especially evident in the FSC channel. You get much better signal to noise ratios and the major parameter that is affected is the laser focusing lens on the stream. Good luck and feel free to contact me if you need more information. Glenn MIT >Dear all, > >I have an enquiry with regards to the flow check paticles that are used for >assessing the optical alignment of the flow cytometer. > >In our work, we are dealing with bacteria of a size range as small as 0.5um. >The flow check we are currently using is the 10um Flow Check Fluorosphere >from Coulter. Noting the relatively large difference in size of the flow >check particles and the cells that we are analysing, does this introduce a >significant amount of error in the sense that the instrument may not be >optimally aligned fot the analysis of such small paticles? > >Thank you for your help. > >Kind Regards > >Neo Sim Yee, Lezlie >Department of Civil Engineering >National University of Singapore
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