Hi Maryalice, Confronted with the problem you describe I'd rather do a (couple?) of cytospins and stain with APAAP for CD30. Then I would also be able to assess if pathological cells are present by morphology. I do not think that there is a great chance to get good results by flow. Best wishes Anna At 16:56 2000-01-04 -0400, you wrote: > Imagine you just receive a CSF and have enough cells to do one tube (no I >don't mean a million cells, I mean thousands). The request indicates the >patient has anaplastic large cell lymphoma and they want you to rule out >CNS involvement. What would you run? What specific panels have you >successfully used to detect anaplastic large cell lymphoma by flow >cytometry (we are experienced with ipox on sections)? I am waiting for >enlightenment so please share your pearls of wisdom. > > Maryalice >Maryalice Stetler-Stevenson >Director Flow Cytometry Unit >Laboratory of Pathology, NCI, NIH Anna Porwit Haematopathology Lab. Department of Pathology Karolinska Hospital, Stockholm anpo@mb.ks.se tel.:+46-851774518 fax.:+46-851775843
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