Re: Clinical case

From: Elisabeth Paietta (epaietta@chcn.org)
Date: Sat Jan 08 2000 - 12:59:50 EST


How sure are you about the kappa/lambda negativity?  Which antibodies do you
use.  How about TdT staining?
EP

Newsom, Brian S. wrote:

> We have a pediatric male patient that presented in July of 1999 with
> neutropenia and a flow phenotype of 12% small (by FSC) cells positive for
> CD10,CD19,CD22, HLA-DRand 50% CD20, K and L negative. He is back in the
> clinic now with the exact same clonal population, still 12% and still the
> same phenotype. I have two questions about this?
>
> 1. Everyone says that hematagones are CD22 negative. Is it possible that
> these are hematagones even though they are strongly CD22 positive?
>
> 2. Are there any additional flow markers that we could use to help define
> these cells and diagnose the patient?
>
> Any help very much appreciated.
>
> Thanks,
> Brian Newsom
> Director, Flow Cytometry
> Center for Cell and Gene Therapy
> Baylor College of Medicine
>
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