Dear flow group, We are currently in the process of evaluating a reliable procedure for CD34 enumeration. We have been sharing samples and comparing results with another institution and have been surprised to find out in the process that lysed specimens yield consistently higher percentages than unlysed specimens. We have been using the Coulter Stemkit which uses CD34-PE/CD45-FITC and their designated lysing solution. The other lab is using a no-lysis procedure and a CD45-FITC/CD34-PerCP combination. I am sure I am not the first one to observe this finding and my questions are : 1. which procedure is recommended these days? 2. which antibody is one supposed to use? Are the ISEHAGE guidelines still recommending CD34-PE? 3. are most people just doing the single parameter CD34 percentage or is the lineage-restricted vs non-restricted %CD34 analysis becoming more important. Thanks for your help and have a happy and safe change into the new millennium! Andrea Illingworth, MS, H(ASCP) Dahl-Chase Diagnostic Services Flow Cytometry 333 State Street Bangor, Maine 04401
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