There are no basophil specific surface markers. There are 2 basophil granule specific markers, BB1 and 2D7, which can be used with I/C staining. . Because of the small numbers of basophils (about 1%) we have generally use 2 markers to identify them. We stain with anti-IgE and a panel of lineage markers (CD2, 14, 16, 19). IgE+, lin- cells are >99% basophils. MHC II positive dendritic cell precursors make up the balance. Calman > ---------- > From: Efros, Lyubov > Sent: Wednesday, March 20, 2002 17:10 > To: Cytometry Mailing List > Subject: Basophills > > > > Dear Flow-ers, > > I am looking if someone have experience using different cell > surface markers for basophils from Human peripheral whole blood ( > without > preparing PBMC). Also, if it possible to do enrichment of > basophils, > without separating mononuclear cells first. > > Any information is greatly appreciated. > > Lyuba Efros > Preclinical Department > PDL, Fremont, CA > lefros@pdl.com > >
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