Depends what you want to do. If you want to look at their function I would use the BASO-test from www.orpegen.de. The kit saves you a lot of hassle with regards to titration and preparation of standards. Regards Gerhard -----Original Message----- From: Efros, Lyubov [SMTP:LEfros@pdl.com] Sent: Wednesday, March 20, 2002 10:11 PM To: Cytometry Mailing List Subject: Basophills Dear Flow-ers, I am looking if someone have experience using different cell surface markers for basophils from Human peripheral whole blood ( without preparing PBMC). Also, if it possible to do enrichment of basophils, without separating mononuclear cells first. Any information is greatly appreciated. Lyuba Efros Preclinical Department PDL, Fremont, CA lefros@pdl.com
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