Folks, I'm seeing a fantastic amount of cell death following a low speed/low pressure sort on my VantageSe and am wondering if anyone has experience with these cells in particular or tips for preventing cell death in general. I'm getting about 10-20% live cell recovery following a typical low pressure sort at 3K/sec, 8 psi, and 18khz with a 100um nozzle. I normally get an average 70% recovery across the board.The cells are P19 embryonic carcinoma cells with CFSE as a marker. They are collected in DMEM with 20% FBS and pen/strep. Thanks. Gene Pizzo/UCONN Health
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