Gene, We are not and have not had this problem with our SE. Perhaps it is the CFSE treatment or the cell lines. Have you run the cell line and sorted for just size or for a different marker? Randy T. Fischer NIH/NIAMS Autoimmunity Branch Building 10, Rm 6D57 9000 Rockville Pike Bethesda, MD 20892 (301) 594-3537 fischer1@mail.nih.gov > ---------- > From: Pizzo,Eugene > Sent: Thursday, March 21, 2002 4:48 PM > To: Cytometry Mailing List > Subject: P19 cell death > > > Folks, > > I'm seeing a fantastic amount of cell death following a low speed/low > pressure sort on my VantageSe and am wondering if anyone has experience > with these cells in particular or tips for preventing cell death in > general. > I'm getting about 10-20% live cell recovery following a typical low > pressure sort at 3K/sec, 8 psi, and 18khz with a 100um nozzle. > I normally get an average 70% recovery across the board.The cells are P19 > embryonic carcinoma > cells with CFSE as a marker. They are collected in DMEM with 20% FBS > and pen/strep. > Thanks. > > Gene Pizzo/UCONN Health > >
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