I have used PE-labeled anti-human CD25 antibody from two biovendors (PharMingen and DAKO) to stain the same peripheral blood lymphocytes by flow cytometry and get completely different result. Both ab recognize 55kD alpha chain of IL-2 receptor. PharMingen said that their CD25 ab should stain about 20% of lymphocytes in normal while DAKO said that their CD25 ab should reacts with less than 5% of lymphocytes in normal human blood. Does anyone know which is correct or any suggestions? Thank you. Danhua Wang MidAmerica Neuroscience dwang@cinpc.com
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