We typically donšt see this with the combinations that we use.
John
On 2/13/02 10:23 AM, "McCloskey, Thomas" <thomasm@nshs.edu> wrote:
>> ----Original Message-----
>> From: Michal Abel [mailto:abelm@necker.fr]
>> Sent: Tuesday, February 12, 2002 5:16 PM
>> To: Cytometry Mailing List
>> Subject: Anti-CD3 and tetramer staining
>>
>> Does anyone know if there is any inhibition of specific tetramer HLA staining
>> with anti-CD3 Ab (as lying closely in the immunological synapse)
>>
>> Hi Michal,
>>
>> There is published evidence for this:
>>
>> Hoffmann et al, Competition of peptide MHC class I tetrameric complexes with
>> anti cd3 provides evidence for specificity of peptide binding to the tcr
>> complex, Cytometry 41:321-328, 2000.
>>
>> Regards,
>> Tom
>>
>>
>>
>>
>>
***************************************************************************
John D. Altman, Ph.D.
Emory University Vaccine Center at Yerkes
954 Gatewood Road
Atlanta, GA 30329
Office: (404) 727-5981
FAX: (404) 727-9005
FAX2: (404) 727-8199
Lab: (404) 727-8914
email: altman@microbio.emory.edu
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Tetramer Core Facility:
http://www.niaid.nih.gov/reposit/tetramer/index.html
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