Looks like it should NOT work. Essentially no absorbance of either the calcium-bound or calcium-free forms of indo-1 at 407 nm Absorption and fluorescence emission (excited at 338 nm) spectra of Ca2+-saturated (A) and Ca2+-free (B) indo-1 (I-1202) in pH 7.2 buffer. [Spectra: 16KB] Marty Bigos wrote: > I know it looks fairly bleak from the excitation/emission spectra, > but i wonder if anyone has had success(or failures!) using indo-1 > excited by the 407 nm krypton line. Thanks for any information on > this. > > Marty > -- > Marty Bigos > Director, Flow Core > Gladstone Institute of Virology and Immunology > Building 3 SFGH Rm 509 > 415-695-3832
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