Hi, everyone: I'm running an EPICS Elite with a gated amplifier and 5 PMTs. We have 3 lasers, one of which is an old Coherent Innova 90 which we have been using for its UV wavelengths. Unfortunately, it has been running "over pressure" for some time, and now gives me an "under voltage" error message. It now will not operate in "light regulation" mode, so I think that it's usefullness as a UV source is at an end. So, what to do now (with a limited budget)? I've heard that there are air-cooled UV lasers now available. Do any of you have experience using one of these for flow? Are they adequate for doing calcium studies with indo-1 or DNA with Hoechst 33342? What manufacture/model do you recommend? Or, are water-cooled lasers still preferred for flow work? If so, please give me your recommendation for getting the plasma tube replaced in our old Innova 90. (Or is there some reason not to try to repair the Innova 90?) Thanks in advance for your advice. Rick Meister * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Richard K. Meister Email: meister.1@osu.edu * * The Ohio State University Voice: (614) 292-9716 * * Dept. of Veterinary Biosciences FAX: (614) 292-6473 * * Cytometry Instrumentation Lab * * 1925 Coffey Road * * Columbus, OH 43210 U.S.A. * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
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