Looks good by do not understand the no DMSO tube. Does that mean that the others have DMSO? I would run them all with DMSO treatment so that you can confirm that the FSxSS are the same for all tubes analyzed. Tim Kute "Nunes, Perla" wrote: > > > Please, I need your help. > I am going to be running flow on cells that are synchronized using > nocazadole (diluted up in DMSO). > I will have two time points, 15 hrs with the drug and 24 hours with > the drug. These cells (adherent) will be stained with FITC conjugated > anti-PARP (primary antibody) and then stained with PI. I will be > doing these 2 drug time points on 5 different cell lines. > > The controls that I plan to use are: > -cells with no DMSO > -cells with FITC Ab only > -cells with PI only > -cells with FITC & PI (DMSO only) (no nocazadole) > > What other controls do I need ??? Do I need controls for both time > points ??? PLEASE ADVICE. > Thanks, > > P. Nunes > Charlotte, NC > perla.nunes@carolinashealthcare.org > > > * > ********************************************************************* > This electronic message may contain information that is confidential > and/or legally privileged. It is intended only for the use of the > individual(s) and entity named as recipients in the message. If you > are not an intended recipient of this message, please notify the > sender immediately and delete the material from any computer. Do not > deliver, distribute or copy this message, and do not disclose its > contents or take any action in reliance on the information it > contains. Thank you.
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:59:22 EST