CD69 stainingDr. Reed, Although I only detect CD69 in human cells, I will share some thoughts - first suggestion is to check your mAB crossreacts with your primate. There was a link on the group earlier about a table of crossreacting antibodies.. I lost it, but you can search the archive for it. second suggestion is to make sure your kinetics/high controlls are good.. when I do CD69 detection I use several high controls - 120ng/mL PMA, CD2/CD2R complex (BD) PHA-P (sigma) assuming our cousins' biology is similar to our own, any of these should give you beautiful CD69 upregulation on any fluor within 3-5 hrs peak with drop after about a day. I did this with and without permeabilization. When I say upgregulation I'm talking about going from 0-1 log to 2-4 log. Let me know if you have other questions about CD69, I use it VERY frequently. Maciej Simm ----- Original Message ----- From: Reed, Doug S Dr USAMRIID To: Cytometry Mailing List Sent: Wednesday, January 30, 2002 1:19 PM Subject: CD69 staining I've got a question for the list regarding CD69 expression, or rather the inability on our part to detect it.
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