I don't know why you may be having a problem with PE. I have used FITC and APC conjugates successfully with 7-AAD and Pyronin Y. ron on 1/28/02 4:02 PM, Newsom, Brian S. at BSNEWSOM@txccc.org wrote: > > Question to all of you DNA experts. We have someone who is tring to run an > experiment staining a nuclear localized protien with PE and then staining > with 7-AAD. The 7-AAD staining works well with a good CV. The antibody > staining looks decent (although low percentage and fairly dim) when by > itself, but when the 7-AAD is added the antibody staining totally goes away. > Is there an energy transfer or steric hinderance issue that may be going on? > Any help appreciated. > > Brian > Ronald L. Rabin, M.D. Senior Staff Fellow Laboratory of Immunobiochemistry DBPAP/OVRR Center for Biologics Evaluation and Research U.S. Food and Drug Administration 29 Lincoln Drive (MSC-4555) Building 29, Room 129 Bethesda, MD 20892-4555 phone: 301.496.8806 fax: 301.402.5177 email: rr84g@nih.gov
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