7-AAD/PE problem

From: Newsom, Brian S. (BSNEWSOM@txccc.org)
Date: Mon Jan 28 2002 - 16:02:03 EST 

Question to all of you DNA experts. We have someone who is tring to run an
experiment staining a nuclear localized protien with PE and then staining
with 7-AAD. The 7-AAD staining works well with a good CV. The antibody
staining looks decent (although low percentage and fairly dim) when by
itself, but when the 7-AAD is added the antibody staining totally goes away.
Is there an energy transfer or steric hinderance issue that may be going on?
Any help appreciated.

Brian

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