Hi everybody,
we have a BD FACS-Vantage and have tried to measure
Ca-fluxes in GFP overexpressing cells. The cells used are
a low-adherent tumor cell line and were detached by
little mechanical force (tapping the flask). We used INDO
as the Ca-dependent fluorescent dye.
Unfortenately, we had bad luck. Even with ionomycin, we did
not get a Ca response.
Has somebody experience with this combination (GFP INDO)
and can help us?
A second question:
Who knows a flow cytometry company working about blood
homeostasis (platelet activation ...) in Europe?
Thanks in advance
Andreas
PD Dr. Andreas Simm
Universitaet Halle Wittenberg
Klinik fuer Herz- und Thoraxchirurgie
Ernst-Grube Str. 40
D-06120 Halle
Tel.: +49 (0) 345 557 2647
552 2878
FAX: +49 (0) 345 557 2782
552 2890
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