In my humble opinion, it is impossible to tell dims from brights unelss both are present. If there is one smear of positive cells spanning a large log range (ex. CD69 expression, inf-gamma etc) that ranges from isotypic cutoff to high 3rd log, I would not call part of that dim and the other bright. I would only talk about dim and bright in cases like CD8 or CD56 on lymphs - where both populations are usually present and can be clearly distinguished on a histogram. Maciej > 2. How / What are the criteria to define the dim, intermediate or > strong antigenic expression? For example: dim for those overlapping > the isotopic cutoff, intermediate for those behind the isotopic > cutoff and strong for those with 2 log scale of the isotopic > control. ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Make a great connection at Yahoo! Personals. http://personals.yahoo.com
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