Dear Flow-ers, Could you kindly give some opinions on the following question? 1. What are the significants of quantitative analysis of antigen expression (e.g. for those cases of immunophenotyping of malignant haematological diseases? 2. How / What are the criteria to define the dim, intermediate or strong antigenic expression? For example: dim for those overlapping the isotopic cutoff, intermediate for those behind the isotopic cutoff and strong for those with 2 log scale of the isotopic control. As coded in Haematologic 2001; 86:675-692, they suggested to use median fluorescence intensity (MFI). Any idea? Best Regards, W.K. LEE United Christian Hospital, Hong Kong, China.
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