A colleague is transplanting human cells into mice and would like to be able to identify, using immunofluorescence and flow cytometry, the transplanted human cells in tissues isolated from the mice. Can anyone recommend monoclonal antibody conjugates that will stain human, but not mouse cells? The human cells are not of hematopoietic origin. Also, because cell surface markers are unlikely to survive the cell isolation procedure, we would prefer an anti-human antibody that reacts with an intracellular antigen, although we wouldn't rule out testing mAb that bind cell surface markers. Any suggestions or comments based on experience with human cell-into-mouse transplants would be much appreciated. Thanks. Gib Otten Chiron Corp. Emeryville, CA 94608 mailto:Gillis_Otten@chiron.com ** ** ATTENTION! This message contains information (including any appended information, such as copied or forwarded messages and attachments, and any message to which this is appended) which may be confidential and/or privileged. Unless you are the addressee (or authorized to receive for the addressee), you may not use, copy or disclose to anyone the message or any information contained in the message. If you have received the message in error, please advise the sender by reply e-mail, and delete the message. Thank you for your assistance. ** **
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