We transplant mice with human cells. The quickest way to determine what is what is to stain for Anti Human CD45 PE and an Anti Mouse CD45 FITC. Jim > ---------- > From: Otten, Gillis > Sent: Monday, September 17, 2001 5:21 PM > To: Cytometry Mailing List > Subject: human cell markers > > > A colleague is transplanting human cells into mice and would like to be > able > to identify, using immunofluorescence and flow cytometry, the transplanted > human cells in tissues isolated from the mice. Can anyone recommend > monoclonal antibody conjugates that will stain human, but not mouse cells? > The human cells are not of hematopoietic origin. Also, because cell > surface > markers are unlikely to survive the cell isolation procedure, we would > prefer an anti-human antibody that reacts with an intracellular antigen, > although we wouldn't rule out testing mAb that bind cell surface markers. > Any suggestions or comments based on experience with human cell-into-mouse > transplants would be much appreciated. Thanks. > > Gib Otten > Chiron Corp. > Emeryville, CA 94608 > mailto:Gillis_Otten@chiron.com > > > ** ** ATTENTION! This message contains information (including any appended > information, > such as copied or forwarded messages and attachments, and any message to > which this is > appended) which may be confidential and/or privileged. Unless you are the > addressee (or > authorized to receive for the addressee), you may not use, copy or > disclose to anyone the > message or any information contained in the message. If you have received > the message > in error, please advise the sender by reply e-mail, and delete the > message. Thank you > for your assistance. ** ** > >
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