RE: human cell markers

From: Houston, Jim (Jim.Houston@stjude.org)
Date: Wed Sep 19 2001 - 08:34:40 EST


We transplant mice with human  cells.  The quickest way to determine what is
what is to stain for Anti Human CD45 PE and an Anti Mouse CD45 FITC.

Jim

> ----------
> From:		Otten, Gillis
> Sent:		Monday, September 17, 2001 5:21 PM
> To:	Cytometry Mailing List
> Subject:	human cell markers
>
>
> A colleague is transplanting human cells into mice and would like to be
> able
> to identify, using immunofluorescence and flow cytometry, the transplanted
> human cells in tissues isolated from the mice.  Can anyone recommend
> monoclonal antibody conjugates that will stain human, but not mouse cells?
> The human cells are not of hematopoietic origin.  Also, because cell
> surface
> markers are unlikely to survive the cell isolation procedure,  we would
> prefer an anti-human antibody that reacts with an intracellular antigen,
> although we wouldn't rule out testing mAb that bind cell surface markers.
> Any suggestions or comments based on experience with human cell-into-mouse
> transplants would be much appreciated. Thanks.
>
> Gib Otten
> Chiron Corp.
> Emeryville, CA  94608
> mailto:Gillis_Otten@chiron.com
>
>
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