Bacterial Counts on Flow

From: Amy Nield (anield@cpd2.usu.edu)
Date: Mon Sep 17 2001 - 12:40:07 EST 

Hi Flowers,

    I have a question that I hope I can get some help with.  We have a client that would like to use the Flow to get an accurate count on his bacterial populations.	He has a kit that he got through Molecular Probes, that stains DNA based upon if the cell membrane is intact or not.  Basically he would like to count the live cells vs. the dead cells.	When we attempted the kit we could not get any separation of live vs. dead cells.  Any help would be greatly appreciated.  If anyone knows of this kit (BacLight Bacterial Viability Kits) and how it works please let me know.

Thank you for your help,

Amy Nield

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