In what sense are you studying activation? If it is in vivo activation, I recommend CD64 (both monocyte and PMN upregulate secondary to INF-gamma, G-CSF and other cytokines) and CD163 (modulated on monocytes by glucocorticoids and cytokines). Regards, Bruce H. Davis, M.D. Maine Medical Center Research Institute 81 Research Drive Scarborough, Maine 04074 USA PHONE: 207-885-8113 FAX: 207-885-8110 Email: davisb@mmc.org >>> Rekha Bansal <bansalr@Gliatech.com> 09/12/01 09:30AM >>> Hi, I am new in this field and interested in studying neutrophil, monocyte, and platelet activation. To prepare and stain cells what do you think would be a good method of choice. Also would like to know what CD markers are specific to each of these cell types. For neutrophils I am using CD15/CD11b, for monocytes I am using CD14/CD11b, and for platelets I am using CD42a and CD62P. Are there more specific CD markers available?. I do staining in PBS,1%BSA, 0.1% sodium azide, 20 mM EDTA. (I added EDTA to prevent further complement activation). Would EDTA in anyway compromise the staining? What G forces are optimal for each of the cell types: I am currently using the following neutrophils/monocyte (300 or 500 g), platelets (1200 g). Rekha Bansal, Ph.D.
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