Hi, I am new in this field and interested in studying neutrophil, monocyte, and platelet activation. To prepare and stain cells what do you think would be a good method of choice. Also would like to know what CD markers are specific to each of these cell types. For neutrophils I am using CD15/CD11b, for monocytes I am using CD14/CD11b, and for platelets I am using CD42a and CD62P. Are there more specific CD markers available?. I do staining in PBS,1%BSA, 0.1% sodium azide, 20 mM EDTA. (I added EDTA to prevent further complement activation). Would EDTA in anyway compromise the staining? What G forces are optimal for each of the cell types: I am currently using the following neutrophils/monocyte (300 or 500 g), platelets (1200 g). Rekha Bansal, Ph.D.
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