Re: Mouse Splenocytes

From: Mark Kukuruga (kukuru@med.umich.edu)
Date: Fri Apr 20 2001 - 23:01:23 EST


Anjli,
Most likely . . . the population to the left (smaller) is dead.  Confirm this by adding
PI to your sample, and look for PI staining.  We looked at "smashing" (assumed, between
slides) vs. "teasing" apart against a wire mesh . . . we found that viability was far
lower in the smashed organ.
MAK.

>>> "anjli kukreja" <anjlik@hotmail.com> 04/20/01 19:08 PM >>>



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