I, too, concur with MAK that one of the populations probably represents dead cells. However, I just want to add that, in our hands, smooshing a mouse spleen between the frosted ends of two microscope slides is much gentler than teasing it apart against a stainless steel mesh. In fact, we routinely prepare mouse spleen cells using slides, with no more than 2-5% dead cells. Julie Oughton Oregon State University Anjli, Most likely . . . the population to the left (smaller) is dead. Confirm this by adding PI to your sample, and look for PI staining. We looked at "smashing" (assumed, between slides) vs. "teasing" apart against a wire mesh . . . we found that viability was far lower in the smashed organ. MAK.
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