I have observed similar multiple populations on FS vs. SSC on a Coulter Elite in mouse lymph node cells so it is probably not your instrument. The conservative course is to include both groups in your 'lymphocyte' gate. In the interests of not losing anything, I tend to gate out the debris and collect everything else. In LN cells, I have seen some markers segregate with a scatter population so excluding one or the other might not be the optimal course. This is just another aspect of mice not being small people. -Jim Weaver ************************************************* * * * James L. Weaver Ph.D. * * Division of Applied Pharmacology Research * * Office of Testing & Research * * CDER MOD-1, FDA * * 8301 Muirkirk Rd, Laurel MD 20708 * * * * Phone: 301-827-8237 * * Fax: 301-594-3037 * * Email:WEAVER@CDER.FDA.GOV * * * *************************************************
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