Try this link. http://facs.scripps.edu/spectra/ Jim Houston -----Original Message----- From: Sathi [mailto:sathi@repromedix.com] Sent: Friday, January 12, 2001 2:01 PM To: cyto-inbox Subject: APC-conjugated antibodies I like to do a 4 color-analysis on Coulter-Epics/XL for lymphocytes. For one of the marker, I have an APC-conjugates antibody. Could any one tell me the emission/excitations spectrum for APC-fluorochrome and which channel should I use for APC on Coulter Epics/XL cytometer. I will be using FITC and PE for other channels and the info would help me to choose the fourth color (ECD or PC-5). I would appreciate any helpful comments. Thanks Sathi ------------------------------------------------------------- T. Sathiyaseelan, B.V.Sc., Ph.D. Repromedix Corp. sathi@repromedix.com
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