Dear Sathi You cannot use APC on the Epics XL. APC requires a red laser source which the XL does not have. Standard set-up of the XL allows use of FITC, PE, PE-Texas Red (ECD)and Pe-Cy5 in that order. There are a number of companies now selling Pe-Texas Red conjugated antibodies. Len Brown MMRI Brisbane, Australia -----Original Message----- From: Sathi [mailto:sathi@repromedix.com] Sent: Saturday, January 13, 2001 6:01 AM To: cyto-inbox Subject: APC-conjugated antibodies I like to do a 4 color-analysis on Coulter-Epics/XL for lymphocytes. For one of the marker, I have an APC-conjugates antibody. Could any one tell me the emission/excitations spectrum for APC-fluorochrome and which channel should I use for APC on Coulter Epics/XL cytometer. I will be using FITC and PE for other channels and the info would help me to choose the fourth color (ECD or PC-5). I would appreciate any helpful comments. Thanks Sathi ------------------------------------------------------------- T. Sathiyaseelan, B.V.Sc., Ph.D. Repromedix Corp. sathi@repromedix.com
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