Sathi, The 4-color XL uses 530, 575, 610, and 675nm bandpass filters. APC excites at 633 nm (HeNe), and emits at ~675 nm . . . the XL won't do it. The 4 color setup typically used on an XL would be: FL1 - - some form of fluorescein FL2 - - some form of phycoerythrin (PE) FL3 - - a PE-Texas Red tandem (Red613, ECD) FL4 - - a PE-Cy5 tandem (Cychrome, TriColor, Red670). MAK. -- Mark A. KuKuruga, Managing Director University of Michigan Flow Core 7416 CCGC 0946 (734) 647-3216, fax (734) 936-7376 kukuru@umich.edu >>> Sathi <sathi@repromedix.com> 01/12/01 03:01PM >>> I like to do a 4 color-analysis on Coulter-Epics/XL for lymphocytes. For one of the marker, I have an APC-conjugates antibody. Could any one tell me the emission/excitations spectrum for APC-fluorochrome and which channel should I use for APC on Coulter Epics/XL cytometer. I will be using FITC and PE for other channels and the info would help me to choose the fourth color (ECD or PC-5). I would appreciate any helpful comments. Thanks Sathi ------------------------------------------------------------- T. Sathiyaseelan, B.V.Sc., Ph.D. Repromedix Corp. sathi@repromedix.com
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