Hello all, There are several things I'm trying to troubleshoot and/or improve and I would appreciate any input from my fellow flowers. Are any of you doing platelet hyper aggregation studies with flow? Also, have you had any experience running CD 38 on CLL patients? It's supposed to be a prognostic indicator. One of our heme onc docs is interested in it. On CLL patients that don't express light chains, do you routinely run them intracellularly? What rate of success do you have with that? Final question. Do you run your light chains in two separate tubes? Is their a reason for that? We are having problems with our three color (kappa-lambda-CD 19) tube and I'm wondering if I should be running two color instead. Thanks for any input. Have a great weekend! Melanie O'Donahue, MT, ASCP Mayo Clinic Scottsdale
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