Dear Melanie, We routinely run a tube with k/l//CD19/CD38 and it affords us several advantages. The K/L is a premixed antibody supplied by Biosource (polyclonal). The CD19 is conjugated with PerCP-Cy5 which is nice because it doesn't cross react with the APC red diode laser. By viewing the K/L together you can more easily differentiate autofluorescence from true clonality that can be a frequent problem. Also, compensation factors arrise when the titer of one is too different from the other. The CD38 is in APC which allows us to see the monoclonal CD38 population as well as any CD19+'s. If the light chain restriction is not distinct we usually follow up with a monoclonal (instead of a polyclonal) kappa/lambda/CD 19 from a different vendor. BD makes a nice one. We have been using this setup for about 2 years and find it to be very useful. Hope this helps, Bruce Greig, Immunopathology Lab Vanderbilt Univ. Medical Center Nashville, TN
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