Adherent cell detachment and adhesion molecules

From: Tomas Corcoran (mascor@gofree.indigo.ie)
Date: Fri Dec 08 2000 - 11:31:06 EST

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Can anyone  tell me how best to detch a microvascular endothelial cell line from 12 well plates. I need to measure adhesion molecules ( ICAM , PECAM , VCAM ) and selectins by flow cytometry and I have had conflicting reports saying that trypsin should not be used for detachment from the plates as it may dirupt the molecules . 
 Can EDTA be used , and will the fact that it's pH needs to be 8.0 to stay in solution have any effect ?

Please help !!!

Tomas Corcoran

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