best 4-color combinations

From: Avis Peters (avispeters@netscape.net)
Date: Mon Nov 06 2000 - 10:30:33 EST


We are looking into performing 4-color immunophenotyping analysis
(leukemia/lymphoma) that must be compatable on a Coulter Elite and BD Calibur.
We want to use FITC,PE, and APC and are wondering which would be the best 3rd
color-PE/Cy7, Percp/Cy5.5, APC/Cy7, ECD, or other fluorochomes? We've heard
about fluorochomes that have varying intensities depending on which CD they
are tagged with and other fluorochromes that bind non-specifically to
monocytes. Which ones cause these problems? We would appreciate any input on
this matter.
Thank you in advance.
Avis Peters
United Hospital
St. Paul, MN

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