Clumping cells

From: Jeff Barry (JBarry@picr.man.ac.uk)
Date: Mon Oct 23 2000 - 08:33:44 EST

Next message: Richard Allman: "software compensation"
Previous message: KAUR, INDRESHPAL (JSC-SD): "(no subject)"
Next in thread: 'FR' Frank Radella II: "Re: Clumping cells"
Reply: 'FR' Frank Radella II: "Re: Clumping cells"
Reply: Derek Davies: "Re: Clumping cells"
Reply: Tom Mc Closkey: "Re: Clumping cells"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Dear Flow People,

A colleague is trying to look for apoptosis using the sub G1, Propidium
Iodide, method however, his cells stubbornly refuses to form single cells
and continue to aggregate causing our FacsCalibur to block every time we put
the samples on. Can anyone relieve our misery by suggesting any sample
preparation tips that will prevent cell clumping.

Regards,

Jeff Barry
Paterson Institute
Manchester
UK

Next message: Richard Allman: "software compensation"
Previous message: KAUR, INDRESHPAL (JSC-SD): "(no subject)"
Next in thread: 'FR' Frank Radella II: "Re: Clumping cells"
Reply: 'FR' Frank Radella II: "Re: Clumping cells"
Reply: Derek Davies: "Re: Clumping cells"
Reply: Tom Mc Closkey: "Re: Clumping cells"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:56:10 EST