Re: Clumping cells

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Keepings cells suspended in 1 to 5 % BSA/media helps prevent clumping.

On Mon, 23 Oct 2000, Jeff Barry wrote:

>
> Dear Flow People,
>
> A colleague is trying to look for apoptosis using the sub G1, Propidium
> Iodide, method however, his cells stubbornly refuses to form single cells
> and continue to aggregate causing our FacsCalibur to block every time we put
> the samples on. Can anyone relieve our misery by suggesting any sample
> preparation tips that will prevent cell clumping.
>
> Regards,
>
> Jeff Barry
> Paterson Institute
> Manchester
> UK
>
>

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