As an addition to the current debate regarding offline colour compensation, We have had difficulty in the past compensating samples on our FACScan, such as some annexin experiments where the green FITC signal may be substantially brighter than the PI signal. In order to address this, we are currently testing a prototype program that we have developed for multicolour compensation using similar algorithms to those described in Bagwell & Adams (1993), Annals New York Academy Sciences 677: 167-184. This is currently a DOS-based program, but we may upgrade it to run in Windows format. Once it is fully tested we expect to make this program available as shareware. Richard Allman Velindre Hospital Whitchurch Cardiff CF14 2TL Wales UK
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