Andrew Beernink writes- >Can somebody tell me how plarization of lasers impacts flow? Which is >recommended? For a comprehensive discussion of that topic - which should be required reading for everybody on the Cytometry Mailing List - see Asbury, Uy, and van den Engh, Cytometry, 2000, 40:88-101. To quote from that paper, "With anisotropy, two instruments can yield different results, and both can be right". However, there is no reason for widespread panic just yet; the flow cytometer manufacturers usually put the lasers in the same way in most instruments, i.e., vertically polarized in the direction of flow, because this orientation is necessary in order to get detectable orthogonal scatter signals. Differences in fluorescence response would be most likely to be seen in instruments with substantially different geometries in the fluorescence collection optics. But you should read the paper. -Howard
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