Re: Monobromobimane Staining?

From: FACS_COPY@wehi.edu.au
Date: Sun Aug 13 2000 - 21:01:40 EST


Tyler,

> We were wondering if anyone has experience with cell surface staining for
> thiols. If you have developed a successful protocol for such a stain, ie
> using MBB, please us know. Thanks!

Take a look at Denizot et. al. PNAS 83(16) 6089 1986.  In that case we
were only looking to track the cells.  The protocol was pretty
straightforward.

Frank Battye.


Frank Battye,        \    /       < The Cytometry Laboratory
Dora Kaminaris,       \__/   <<<<<  The Walter & Eliza Hall Institute
Viki Lapatis,    ------!!<<<<<<<<   Victoria  3050,   AUSTRALIA
Jennie Chan and       /!!\   <<<<<  ph: 61_3_9345 2540 fax: 61_3_9347 0852
Catherine Tarlinton  o !! \       < in::   "facs_copy@wehi.edu.au"



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