Warren, I recently read in a Carleton and Sigrid Stewart article that the most common cause of the delay calibration failure is the low sheath fluid tank volume. That is a plastic tank and I assume when filled with air the pressure is lower than when it's full of liquid. We always fill up the tank before four color runs and don't let it drop below half the volume. Akos Akos Szilvasi Biogen Cambridge, MA "Warren D. Shlomchik" <wds8@email.med.yale.edu> on 06/26/2000 05:07:39 PM To: cyto-inbox cc: (bcc: Akos Szilvasi/Cambridge/Biogen) Subject: problems with time delay calibration on FACS Calibur Dear Flowers (in particular FACS Calibur users), We have a new FACS Calibur and have had persistent problems with reproducibly being able to set time delay calibration. In particular, we get the error message, "signal out of range...unable to set time delay." BD has replaced an EPROM and a board, but the problem intermittently persists. In speaking to users of 3 other Caliburs I discovered that they too had seen this problem. I'd appreciate any comments from other two laser users on the Calibur and will summarize the responses to the list. Many thanks. Warren Warren D. Shlomchik, M.D. Assistant Professor of Medicine Yale University School of Medicine Section of Medical Oncology 333 Cedar Street WWW217 New Haven, CT 06520 phone:(203)737-2478 FAX: (520)395-8571
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:55:55 EST