Hi, a little off the flow topic (but only a little).... we are doing quantitative PCR on very small numbers of sorted cells, and are looking around for a different housekeeping gene for control purposes than beta-actin. We need one with an intron so that we can differentiate cDNA from genomic in our ultrasensitive amplifications. For people who are using housekeeping RNA's as controls, I'd like to get feedback: what gene products do you measure? Do you take advantage of spanning a splice junction? Have you measured the variation in different cell types or stimulation conditions? Thanks for any info mr
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