Mario Roederer wrote: > Hi, a little off the flow topic (but only a little).... > > we are doing quantitative PCR on very small numbers of sorted cells, > and are looking around for a different housekeeping gene for control > purposes than beta-actin. We need one with an intron so that we can > differentiate cDNA from genomic in our ultrasensitive amplifications. Since you're using PCR, just design your primers so they span adjacent exons. This way, genomic DNA won't amplify and your only signal will be the cDNAs. -- Tony Schountz, Ph.D. Department of Biological Sciences Mesa State College mailto:tschount@mesastate.edu
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