Greetings once again, We have recently received a blood specimen and were asked to stain for CD18/11b ( I love it how doctors don't explain the condition of the patient ahead of time, so we know what to look for ) We found, that when compared to our NC's (N=300+ for this "tube" and growing) this patient had less +/+ lymphoctes (~30% vs. 12%). The expression of CD18 was not reduced - all of his leukocytes had >97%. A question came up: what is the significance of CD18+/CD11b- lymphocytes? what is the significance of a decrease in the +/+ population? We do not routinely do CD11a or CD11c - are these mutually exclusive? I.e. it's either a, b or c but never any of them together? And finally, is the ligand for CD11b soluble and may have interfered with our staning? This staining was done in whole blood (which I just found is a no-no). I would appreciate if anyone could share any thoughts on this topic. Maciej __________________________________________________ Do You Yahoo!? Send online invitations with Yahoo! Invites. http://invites.yahoo.com
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:55:45 EST