I found that what fixative makes a difference. (Beckman)Coulter's Permafix preserved CD34 antigenicity for one Ab, while BD's FACS Lysing Solution destroyed it. A different Ab didn't work with either fixative. (N = 1) At 04:04 PM 4/17/00 -0300, Olindo Assis wrote: >Dear Irene, > >I have used for human cells, anti-CD4 and anti-CD8 FITC labeled (from BD, >Immunotech, Dako, Diatec and Pahrmingen) without any problem to detect cell >surface marker after fixation. However, I know that anti-CD14, anti-CD19 and >anti-CD16 does not recognize fixed cells. > >Best regards, > >Olindo > > --- Dennis Dennis J. Young Mail:<<mailto:djyoung@ucsd.edu>> WWW:<<http://cancer.ucsd.edu/SResources/flow.htm>> Flow Cytometry Core Facility University of California, San Diego Internal Medicine Group, Bldg #4, Rm 126 9500 Gilman Drive La Jolla CA 92093-0671 Telephone:(858) 822-0407
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