Re: putting surface markers in fixed cells

From: Olindo Assis (oamfilho@cpqrr.fiocruz.br)
Date: Mon Apr 17 2000 - 14:04:32 EST

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Dear Irene,

I have used for human cells, anti-CD4 and anti-CD8 FITC labeled (from BD,
Immunotech, Dako, Diatec and Pahrmingen) without any problem to detect cell
surface marker after fixation. However, I know that anti-CD14, anti-CD19 and
anti-CD16 does not recognize fixed cells.

Best regards,

Olindo

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