putting surface markers in fixed cells

From: Irene_Tham (Irene_Tham@hms.harvard.edu)
Date: Fri Apr 14 2000 - 15:29:17 EST 

dear flowers,

once cells are stained with surface markers and fixed, can you put
additional surface markers back into the same sample for detection?  i was
told that you can with some Ab. if so, which Ab? Can one reverses the
effect of paraformaldehye? Any suggestions will be appreciated.

irene

			       Irene C. Tham
		       Harvard Medical School - NERPRC
		     Department of Comparative Pathology
			     1 Pine Hill Drive
			  Southborough, MA 01722
			   Office: 508-786-1412
			     Lab: 508-624-8051
			     Fax: 508-624-8181

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